High-throughput single-cell omics using semipermeable capsules
Summary
Biological systems are inherently complex and heterogeneous. Deciphering this complexity increasingly relies on high-throughput single-cell omics methods and tools that efficiently probe the cellular phenotype and genotype. Here, we present a versatile technology based on semipermeable capsules (SPCs) tailored for a variety of high-throughput nucleic acid assays, including single-cell genome and mRNA sequencing, and fluorescence-activated cell sorting-based isolation of individual transcri
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# High-throughput single-cell omics using semipermeable capsules
*Published: 2026 Mar 12*
Biological systems are inherently complex and heterogeneous. Deciphering this
complexity increasingly relies on high-throughput single-cell omics methods and
tools that efficiently probe the cellular phenotype and genotype. Here, we
present a versatile technology based on semipermeable capsules (SPCs) tailored
for a variety of high-throughput nucleic acid assays, including single-cell
genome and mRNA sequencing, and fluorescence-activated cell sorting-based
isolation of individual transcriptomes based on a nucleic acid marker of
interest. Being biocompatible, the SPCs support single-cell cultivation and
clonal expansion over long periods of time, thereby overcoming a fundamental
limitation of droplet microfluidics platforms. Overall, the SPCs represent a
customizable and broadly applicable tool for easy-to-use, scalable single-cell
omics applications that are built on multistep biochemical reactions.
DOI: 10.1126/science.ady7227