The E3-ome gene-centric compendium reveals the human E3 ligase landscape
Summary
To define and systematically characterize the human E3 ubiquitin ligase (E3) landscape, we generated the E3-ome, a compendium of E3s encoded by the human genome. The E3-ome integrates experimental data, bioinformatics, and published research, revealing 672 high-confidence E3s. We standardized E3 classifications to create a unified framework for annotation and comparative analysis. The E3-ome identified several previously unrecognized domains, motifs, E3 candidates, and relationships, expan
Content
# The E3-ome gene-centric compendium reveals the human E3 ligase landscape
*Published: 2026 Apr 2*
To define and systematically characterize the human E3 ubiquitin ligase (E3)
landscape, we generated the E3-ome, a compendium of E3s encoded by the human
genome. The E3-ome integrates experimental data, bioinformatics, and published
research, revealing 672 high-confidence E3s. We standardized E3 classifications
to create a unified framework for annotation and comparative analysis. The
E3-ome identified several previously unrecognized domains, motifs, E3
candidates, and relationships, expanding the diversity of E3s. Furthermore, the
E3-ome mapped the spatial and physiological organization of E3s across human
tissues and cell types, revealing context-dependent E3s. Genetic analyses
identified disease-associated variants across the E3-ome, linking E3s to diverse
human pathologies. Together, these analyses define the human E3 landscape at
high resolution and deliver a foundational resource to drive mechanistic and
therapeutic discovery.
DOI: 10.1016/j.cell.2026.01.029